1The State Education Institution of Higher Professional Training, The First Sechenov Moscow State Medical University under Ministry of Health of the Russian Federation (Sechenov Univesity), 8-2, Trubetskaya street, Moscow, 119992
2All-Russian Scientific Research Institute of Medicinal and Aromatic Plants, 7, Grina street, Moscow, 117216
To study the composition of phenolic compounds of R. caesius leaves and evaluate of antioxidant, antiinflammatory, antimicrobial activity of the infusion from raw materials.
The objects of the study were the leaves of R. caesius L. collected on the territory of the Moscow region. The composition of phenolic compounds of the leaves was studied by HPLC on a Nexera-i LC-2040 chromatography. To study the antioxidant activity of the infusion from the leaves of R. caesius L., a reaction with the free radical DPPH was used. The study of anti-inflammatory activity was carried out in vivo on 90 non-linear male mice on the formalin-induced edema model. Antimicrobial activity was determined against pathogenic microorganisms: Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Candida albicans and Microsporum canis.
During the HPLC-UV analysis, 7 compounds of phenolic nature were identified in the leaves of R. caesius L.: gallic acid, rutin, cynaroside, narcissin, astragalin, kaempferol, isorhamnetin. When studying antioxidant activity, IC50 values was calculated for infusion of R. caesius L. leaves, which was 43.6±0.4μg/ml. When an infusion of R. caesius L. leaves was administered to animals, a 21% decrease in formalin-induced edema of the paws was observed in comparison with the control group. It was found that the infusion of R. caesius L. leaves has antimicrobial activity against Staphylococcus aureus, Proteus vulgaris, Escherichia coli, Pseudomonas aeruginosa, Microsporum canis and Candida albicans.
The composition and content of phenolic compounds in the leaves of R. caesius L. have been studied. It has been shown that the infusion from the leaves of R. caesius L. have high antioxidant activity even with large dilutions. Based on the conducted studies, it was found that the infusion of R. caesius L. leaves has anti-inflammatory and antimicrobial activity and is a promising pharmaceutical form for further study and use in medicine.
European dewberry, Rubus caesius L., Phenolic compounds, HPLC, Pharmacologic activity
