Development of UV Chemometric Technique for Resolving the Overlapped Spectra of Sacubitril and Valsartan in their Combined Pharmaceutical Dosage Form

A UV spectroscopic technique that indicates stability has been developed and evaluated for the assessment of valsartan (VAL) and sacubitril (SAC) in pharmaceutical dosage forms and bulk. The method’s robustness, accuracy, precision, and linearity were determined, confirming its appropriateness for routine screening. A linear relationship was established over a concentration range suitable for both compounds, with high correlation coefficients. Recovery studies verified the method’s accuracy, with percentage recoveries falling within the accepted range of 98–102%. This method provided intermediate precision and repeatability with minimal relative standard deviations. Results from the approved UV-Visible spectroscopic method were statistically compared with those from the chemometric analysis of spectroscopic data. Principal Component Regression (PCR) was selected due to its ability to handle complex and correlated data sets efficiently by reducing dimensionality, addressing multicollinearity and improved accuracy. In UV method development, PCR is particularly effective for resolving overlapping spectra and distinguishing between components in multicomponent formulations.