In Vitro Evaluation of Anti-Diabetic and Anti-Inflammatory Potential of Lagerstroemia lanceolata Leaf Extract

Lagerstroemia lanceolata Wall., a member of the Lythraceae family, can be found growing from south of Bombay to Kerela, as well as in the hills of the Deccan Peninsula at elevations of up to 1200 meters. Ethanolic extract was used to carry out the in-vitro anti-diabetic activity by α-amylase inhibitory assay, along with % glucose uptake assay in yeast cells and in-vitro anti-inflammatory activity on BSA (Bovine serum albumin) by using protein denaturation assay. In-vitro anti-diabetic activity revealed that the ethanolic extract (LLLE) exhibited inhibition of α-amylase activity in dose dependent manner. LLLE exhibited IC50 value of 101.53μg/ml as compared to standard Metformin (IC50 value=23.91μg/ml). The % of glucose uptake assay in yeast cells revealed that LLLE and standard drug metronidazole at 250μg/ml exhibited maximum % glucose uptake of 67.09% and 87.44% respectively. LLLE has revealed IC50 value 191.42μg/ml while standard Metronidazole exhibited IC50 value of 76.03μg/ml. The antidiabetic activity of the present study may be attributed due to the presence of polyphenols and flavonoids present in LLLE04 and revealed that the ethanolic extract (LLLE) exhibited inhibition of α-amylase activity in dose dependent manner. In-Vitro anti- inflammatory activity revealed that LLLE showed IC50 value 177.07μg/ml as compared to standard aspirin (IC50=78.53 μg/ml) which exhibited moderate protein denaturation activity. The observed pharmacological activities further support its traditional uses and provide a scientific basis for future research and applications.