Analytical Method Development and Validation of Antihypertensive Drug: Rosuvastatin Calcium using Reverse Phase-High Performance Liquid Chromatogrhaphic Method

A liquid chromatography method was developed to estimate Rosuvastatin calcium in bulk and pharmaceutical dosage forms accurately. The developed method demonstrates high accuracy, specificity, and reliability, making it particularly suitable for pharmaceutical research. The chromatographic analysis was performed using a C-18 column (250 × 4.6mm, 5μm) with a mobile phase consisting of Acetonitrile and acidified water (pH 3.0 adjusted with orthophosphoric acid) at a ratio of 55:45 v/v. Detection was achieved at a wavelength of 237nm. The retention time for Rosuvastatin Calcium was observed at approximately 7.0 minutes.