Rhizogenesis Related Biochemical Changes in Callus Cultures of Chlorophytum borivilianum Sant. Et Fernand.

Biochemical changes during in vitro root formation in Chlorophytum borivilianum were investigated in the callus derived from bud pedicel on MS medium supplemented with 4.4 μM BAP + 1.0 mg/l NAA. The callus subcultured on MS medium supplemented with 4.4 μM BAP + 2.69 μM NAA showed roots differentiation within 4–5 days of inoculation. Starch content and reducing sugars were lower while total soluble sugars, free amino-acids, total soluble proteins and total phenols were higher in the roots differentiating callus cultures than in control callus. The activity of enzymes viz. α-amylase, acid-protease, acid-phosphatase and peroxidase increased on 4^th and 5^th day whereas, the acid invertase activity decreased till the appearance of roots indicating their role in the differentiation.