Induced chlorophyll mutations and mutagenic effectiveness in indian mustard (Brassica juncea L. czern and coss)

Two varieties of Indian mustard viz., Krishna and Kranti were treated with different concentration of EMS for different durations. Eleven different types of chlorophyll mutants viz., albina, xantha, viridis, tigrina, maculata, variegated, albo-xantha, xantho-alba, xantho viridis, virido-xantha and virido-alba were isolated with different doses of EMS. Treatment with 0.4% EMS 16 h produced the highest percentage of chlorophyll mutations in both the varieties. Where as the effectiveness of treatment was highest with 0.4% EMS 8h treatment in both the varieties. Results from chlorophyll mutations suggest that long r duration of treatment with higher concentration of EMS is not suitable to generate mutagenic variability.