Bisorption of heavy metals oxides by heavy metals Resis-Tant microorganism in liquid Culture-ICP-MS Technique

The Seven acclimated microorganism Pseudomonas aeruginosa (CCSUPa3), Pseudomonas fluoresencs (CCSUPf4), Escherichia coli (CCSUEc9), Micrococcus luteus (CCSUMl11), Klebsiella pneumoniae (CCSUKp13), Staphylococcus aureus (CCSUSa16) and Bacillus subtilis (CCSUBs17) were isolated from root of Eicchornia crassipes grown at metals pollutant drainage sites of industries under gone enrichment culture techniques. These isolated strains showed highly resistance against metal oxide especially Arsenic trioxide (As2O3), Lead oxides (PbO) and Nickel oxides (NiO) and their resistance capacitance were comparison with three standards MTTC culture such as Pseudomonas aeruginosa (MTCC-647), Micrococcus luteus (MTCC-1538) and Staphylococcus aureus (MTCC-3160). They were allowed to grow in Luria Broth (LB) medium amended with metal oxide the residual heavy metals oxides were determined in liquid culture by advance techniques Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). The acclimated microorganisms were used to remediate the metals oxides by the bisorption process in batch culture. The Pseudomonas aeruginosa (CCSUPa3) were reduced the arsenic trioxide 0.1415mg/liter in 24 hours to 0.20mg/liter in 48 hours, Micrococcus luteus (CCSUMl11) 0.1063 mg/liter in 24 hours to 0.1205 mg/liter in 48 hours, Klebsiella pneumoniae (CCSUKp13) 0.1166 mg/liter in 24 hours to remediate 0.1293 mg/liter in 48 hours, Staphylococcus aureus (CCSUSa16) 0.0798mg/liter in 24 hours to 0.1016 in 48 hours and Bacillus subtilis (CCSUBs17) to remove the heavy metals oxide 0.537 mg/liter in 24 hours to 0.587mg/liter in 48 hours. The capacitance of heavy metals removals were comparison with three standards MTCC culture and results were close similar to field isolated. These isolates further may be used as biological treatment of industrial effluent.