1College of Bio Technology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P.)
2Indian Agriculture Research Institute, New Delhi
Online published on 4 March, 2015.
The genetic variation and relationships among 32 rice genotypes of diverse origin were evaluated using 19 simple sequence repeat (SSR) primers. Among 19 SSRs primers, 17 showed 100% polymorphism. The number of alleles ranged from 2 to 10 with the average value of 4.37 per locus. Three primers generated a total of 5 rare alleles. Polymorphism information content (PIC) values of the primers ranged from 0.79 (RM 167) to 0.99 (RM 47 and RM 10) with an average of 0.84. Cluster analysis based on SSR banding pattern grouped the rice genotypes into 2 major clusters with additional sub-clusters. SSR analysis revealed that genotype AUS287 was distantly related to Kalonchi with Jaccard similarity coefficient of 0.64. SSR analysis resulted in a more definitive separation of clusters of genotypes, indicating a higher level of efficiency of SSR markers for the accurate determination of relationship between genotypes.
Rice, SSR, Genetic Variation, Genotypes
