Phylogenetic relationship as inpalmarosa (Cymbopogon martinii) revealed by RAPD markers

Genetic relationships were examined among sixteen accessions of Palmarosa (Cymbopogon martinii) was estimated using Random Amplified Polymorphic DNA (RAPD) markers. Fifty primers were used in the study of RAPD markers analysis to standardization of suitable specific primers amplifying the genetic materials of Palmarosa accessions. All primers, but (OPA-18, OPD-8, OPF-4, OPF-2 and OPE-19) yielded maximum amplification products with all Cymbopogon martinii accessions. The primers amplified DNA products from each Palmarosa accession generating reproducible band patterns. Analysis of sixteen Palmarosa accessions showed (91.26%) of polymorphism. The total 355 bands were scored for the 50 RAPD primers out of which 31 bands were monomorphic and 324 bands were polymorphic. DNA based markers are very powerful maker to study genetic relationship among the related accessions and varieties. In comparison to other maker they are not affected by the environments and the developmental stage of the individual.