Comparative assessment of ssr markers derived from different sources in genetic diversity analysis of Vigna genotypes

Mung bean (Vigna radiata (L.) Wilczek), also known as green gram, is an important food legume crop and provides an inexpensive source of dietary protein. Due to lack of sufficient variability and its importance in Asian countries there is need of broadening its genetic base. Genetic diversity among 21 Vigna genotypes of mung bean, rice bean and urd bean was assessed using SSR markers derived from genomic sequences of azuki bean (Ced) and mung bean (VR) and EST sequences of mung bean (MBM). Of the 67 SSR markers selected, 45 were found polymorphic among different genotypes which yielded 138 alleles of which 16 were mung bean specific, 27 urd bean specific and 17 rice bean specific alleles. Azuki bean derived SSRs (Ced) were more polymorphic. PIC value was similar in three sets of SSRs. Number of observed and effective alleles were more in Ced markers along with high value of various other parameters like EMR (3.6), MI (1.26), RP (2.7), H(0.33), H(0.62), and H(0.6), calculated to identify effectiveness of markers. Dissimilarity analysis divided mung bean and rice bean genotypes into two subclusters while 12 genotypes of urd bean formed two clusters.