Optimization of conditions for release of protoplasts from oyster mushroom (Pleurotus spp.)

The studies were undertaken for developing an efficient protocol for production and release of protoplasts from two species of Pleurotus viz P. florida (PF-5) and P. sajor-caju (PSC-3) recommended for commercial cultivation by mushroom growers of the State with the objective of using these in systematic strain improvement programme to develop intra/interspecific hybrids. It was found that 3–4 day old mycelium of both the species released maximum protoplasts (1.62 x 10⁷ and 1.67 x 10⁷/ml), using a combination of 0.1% Chitinase, 0.1% Lyticase and 0.5% Driselase in the presence of 0.6M MgSO_4-. 7H₂O at 25°C after 6–12 hours incubation period.