Studies on In vitro Plantlet production in rough lemon (Citrus jambhiri Lush.)

In rough lemon (Citrus jambhiri Lush.) the highest percentage of explant establishment was observed on MS medium supplemented with BAP (1.0 mg/l) + Ads (1.5 mg/l). Maximum shoot proliferation was on MS solid medium containing BAP (1.0 mg/l) + Kin (0.5 mg/l) with vertical orientation of explant. Orientation and phase of media did not significantly affected the number of shoots proliferated per culture. Earliest root initiation and maximum percentage of rooting was observed on MS liquid media supplemented with 1.0 mg/l IBA and NAA each. Maximum number of roots per shoot and root length were observed on solid phase of the same media. Half strength MS salts proved better than quarter and full strength with respect to time taken for root induction, rooting percentage, root number per shoot and root length.