Microbial lipase production in poultry feed ingredients

Rhizopus oryzae was grown on fermentation media containing maize flour, soyabean meal and de-oiled rice bran with or without emulsified olive oil at 30°C. The lipase enzyme activity and total protein concentrations of fermentation media were determined at 24, 48, 72 and 96 h of fermentation. The results revealed that soybean meal produced high lipase activity at 72 h of fermentation compared to de-oiled rice bran (DORB) and maize flour. Addition of olive oil to fermentation media increased the lipase production in all the fermentation media. The total protein assay revealed that soybean meal was having more protein in all fermentation periods compared to DORB and maize flour.