1Ma’mun Academy, Khiva City, Khorezm Region, Zargarlar Street, Uzbekistan
2Ma’mun Academy, Khiva City, Khorezm Region, Zargarlar Street, Uzbekistan
3Urganch State University, Urgench city, Khorezm Region, Hamid Olimjon Street, 220100, Uzbekistan
4Institute of Zoology Academy of Sciences the Republic of Uzbekistan, Tashkent city, Yunusabad District, Bagishamol Str., 232 b, 100053, Uzbekistan
*Email: temuratajanov.92@mail.ru
**Email: manzuradoschanova84@gmail.com
***Email: uduschanov@inbox.ru
Harpalus rufipes De Geer (Coleoptera: Carabidae) is a widespread ground beetle in Uzbekistan and plays an important ecological role in agroecosystems. In this study, 24 mitochondrial COI (mtCO1) sequences (659 bp) were analyzed, revealing a high level of genetic diversity (h = 0.996; π = 0.079). A phylogenetic tree was constructed using the maximum likelihood method, identifying two major clades and several internal subclades, with the Uzbek specimen forming a distinct monophyletic clade. Genetic distances estimated under the Kimura 2-parameter model were minimal within H. rufipes (0.0-0.2%), while interspecific distances ranged between 4.5-7.0%. These results provide important insights into the molecular-genetic characteristics and population-level evolution of H. rufipes.
Harpalus rufipes, Carabidae, COI gene, mitochondrial DNA, genetic diversity, phylogenetics, maximum likelihood, Uzbekistan
