1Department of Entomology, Keladi Shivappa Nayaka University of Agricultural and Horticultural Sciences, Shivamogga577204, Karnataka, India
2Indian Agricultural Research Institute Academic hub, Central Institute for Cotton Research, Nagpur440 010, Maharashtra, India
3Department of Entomology, University of Agricultural Sciences, Bengaluru560065, Karnataka, India
*Email: nikhilreddy1718@gmail.com (corresponding author):
CRISPR-Cas9 is a groundbreaking genome-editing tool that allows precise modifications to DNA. It works by using the Cas9 protein, guided by synthetic RNA, to create double-strand breaks at specific genome locations, which are then repaired through non-homologous end joining or homology-directed repair. CRISPR’s versatility enables the creation of genetically modified organisms and innovative pest management strategies, including gene drive systems that rapidly spread genetic modifications to control pest populations. CRISPR has been used in mosquitoes to combat diseases like malaria and in agricultural pests, offering environmentally sustainable alternatives to traditional pest control methods like chemical pesticides and biological control. Compared to other gene-editing technologies, CRISPR offers several distinct advantages, including its high precision, ease of design and the ability to target multiple genes simultaneously. Its use in pest management holds promise for reducing environmental impact, minimizing off-target effects, and providing long-term solutions to pest-related challenges in agriculture and public health.
Insect pests, Genome editing, CRISPR-Cas9, genetically modified organisms (GMOs), gene drive systems, synthetic RNA, Sterile insect technique (SIT), RNA-guided gene editing, pest management
