1Department of Ecology and Environmental Engineering, Faculty of Natural and Life Sciences and Earth and Universe Sciences (SNVSTU), University 8 Mai 1945, Guelma, BP 401, Guelma, 24000, Algeria
2Faculty of Medicine, University of Badji Mokhtar, Annaba, B.P.12, Annaba, 23000, Algeria
3Department of Biology, University of Badji Mokhtar, Annaba, B.P.12, Annaba, 23000, Algeria
*Email: messiadrouhia@gmail.com; messiad.rouhia@univ-guelma.dz (corresponding author): Orcid Id0000-0002-3980-9611
Online published on 13 March, 2026.
The objective of this study was to estimate insecticidal effects of Urtica dioica among the associated human pest Musca domestica. The toxicity of leaves composition was experimented by ingestion estimating the lethal doses (LD50) which were 2.39, 3.16 and 5.48 mg/ insect) obtained, respectively after 1, 3 and 6 hr. After this preliminary study, LD50 was evaluated, on acetylcholinesterase and glutathione S-transferase. The enzymes specific activity revealed an increase in GST at all tested times 10, 15 and 20 min. However, AChE activity showed significant alterations only after 10 min of exposure comparatively of control.
Musca domestica, Urtica dioica, Biopesticide, Toxicity, LD50, Acetylcholinesterase, Glutathione S-transferase, Integrated pest management
