Indian Journal of Entomology
Open Access
SCOPUSWeb of Science
  • Year: 2022
  • Volume: 84
  • Issue: 1

Genome Size Estimation of Potato Aphid Macrosiphum Euphorbiae Using Flow Cytometry

  • Author:
  • Kailash Chandra Naga*, Hemant B Kardile, Rahul K Tiwari, S Subhash1, Gaurav Verma, Sanjeev Sharma, Anuj Bhatnagar1
  • Total Page Count: 3
  • Published Online: May 16, 2022
  • Page Number: 149 to 151

1ICAR-Central Potato Research Station, Modipuram250110, Uttar Pradesh, India

ICAR-Central Potato Research Institute, Shimla171001, Himachal Pradesh, India

*Email: kailashnaga3j@gmail.com (corresponding author)

Online Published on 16 May, 2022.

Abstract

Potato aphid Macrosiphum euphorbiae (Thomas) (Hemiptera: Aphididae) is colonizing species and vector for many economically important potato viruses. There is dearth of genomic information about this economically important aphid species. Hence, to get insight into the genomic architecture, genome size was determined using flow cytometry. The estimated size of M. euphorbiae was 0.53 pg or 519.4 Mbp. The genome size of M. euphorbiae is approximately 2.9, 2.2 and 1.9x larger than that of Drosophila melanogaster, honey bee (Apis mellifera) and mosquito Anopheles gambiae, respectively. The generated genome size information will provide the foundation for futuristic genomic research on M. euphorbiae.

Keywords

Macrosiphum euphorbiae, Potato, Flow cytometry, Feulgen densitometry, Genome size, Mtcoi, Drosophila melanogaster, Apis mellifera, Anopheles gambiae