1Assistant Professor, College of Veterinary and Animal Sciences, (MAFSU, Nagpur), Parbhani, Maharashtra, India
2Senior Scientist, ICAR–Indian Institute of Millets Research, Hyderabad-50030, Telangana, India
3Livestock Development Officer, Department of Animal Husbandry, Maharashtra, India
4Retired Principal Scientist & National Professor, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India
*Corresponding author E-mail: prabhakar.ghorpade@covaspbn.co.in
Online published on 25 September, 2025.
Methionyl-tRNA synthetase (MetRS) is a single-copy informational gene in Salmonella serovar Typhimurium. Informational genes are more conserved than operational genes. In this study, we analyzed HGT events within MetG sequences of different bacterial genera. A species tree based on 16S rRNA sequences of the same genus was drawn and evaluated against the generally accepted species tree of the bacteria. The MetG phylogenetic tree was evaluated against the 16S rRNA tree, and an HGT event was identified. Similarly, phyla trees were made, and HGT events were identified. Twenty-four HGT events were identified between genera and eleven within phyla. MetG is considered a conserved gene; finding so many HGT events in this gene indicates that horizontal gene transfer is very common in this gene.
Methionyl tRNA synthetase, Horizontal gene transfer, Topological difference, MEGA, T-REX
