1Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshukh Veterinary Science University, Mhow-453 446, Indore, Madhya Pradesh, India
2Project Directorate on Foot-and-Mouth Disease (PD-FMD), Indian Veterinary Research Institute Campus, Mukteshwar, Kumaon-263 138, Nainital, Uttarakhand, India
*Corresponding author E-mail: asd_vet@yahoo.com, audarya.sachin@gmail.com
Online published on 12 August, 2016.
Interleukin-2 (IL-2) plays its role in maturation of immature T cells to functional T cells. This study is planned to standardise polymerase chain reaction (PCR) for amplification of IL-2 gene from bovine peripheral blood mononuclear cells (PBMCs). Blood samples were collected from cattle in containers with 0.1% ethylenediaminetetraacetic acid anticoagulant. PBMCs were isolated by using commercially available Histopaque®-1077. Extracted total ribonucleic acid was utilised in reverse transcription to prepare complementary deoxyribonucleic acid (cDNA). The resulting cDNA was employed in PCR assay. IL-2-specific primer set along with positive control was commercially procured to amplify partial nucleotide sequence of IL-2 gene successfully. The resulting amplified IL-2 DNA product (345 bp) was run on 1% agarose gel electrophoresis and documented by using gel documentation system.
Cytokine, IL-2, Cattle, PCR, PBMCs
