Regional Research Centre on Foot-and-Mouth Disease, Department of Veterinary Microbiology, COVS, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, India
*Corresponding Author: E-mail: rsharma698@gmail.com
Online published on 2 April, 2016.
Foot-and-mouth disease virus (FMDV) was detected from the oropharyngeal fluid (OPF) by reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay, SYBR Green dye based quantitative reverse transcription polymerase chain reaction (qRT-PCR) and conventional virus isolation for identification of carriers in infected bovines in Haryana. Out of the 11 OPF samples collected at 45 days post infection, RT-LAMP assay detected five as compared to three by qRT-PCR and two by virus isolation. Compared with qRT-PCR and virus isolation, RT-LAMP was consistently fast and amplification products were detected by visual inspection and agarose gel electrophoresis. The results indicated the potential usefulness of the RT-LAMP technique as a simple and rapid procedure that can be employed for the detection of FMDV carriers in OPF samples.
Foot-and-mouth disease virus, Persistent infection, Reverse transcription loop mediated isothermal amplification (RT-LAMP), Rapid detection of carriers, Buffaloes
