Bacterial isolates from uterine discharge of bitches and their antibiogram

Study was carried out on 96 swabs of uterine discharge of bitches with a history of infertility and repeat breeding irrespective of their age or breed, received in College Central Laboratory. On bacteriological examination, 92 (95.83%) were found positive for bacterial growth yielding 120 isolates. Of these positive samples, 65 (70.65%) yielded single bacterial species, 26 (28.26%) yielded two species, and the remaining 1 (1.08%) yielded 3 different bacterial species. Of 120 isolates, Streptococcus pyogenes was the predominant organism accounting for 55 (45.83%) infections followed by Staphylococcus aureus 23 (19.16%), Escherichia coli 20 (16.66%), Corynebacterium pyogenes 19 (15.83%), Pseudomonas aeruginosa 2 (1.67%) and Klebseilla pneumoniae 1(0.83%). Most common mixed infection was of S. aureus + S. pyogenes (11) and S. pyogenes + C. pyogenes (7) followed by other combinations S. pyogenes + E. coli (3), C. pyogenes+ E. coli (2), S. aureus + S. pyogenes + C. pyogenes (1), P. aeruginosa + S. pyogenes (1), E. coli + K. pneumoniae (1), S. aureus + C. pyogenes (1). Overall antimicrobial sensitivity pattern revealed that majority of the isolates were highly sensitive (80.20 to 89.58%) for azithromycin, norfloxacin, ceftriaxone, cephaperazone and clindamycin. Whereas 77.08 to 79.16% bacterial isolates were moderately sensitive towards oxytetracycline, chloramphenicol, ciprofloxacin, erythromycin, gentamicin, amoxycillin, carbenicillin. Least sensitivity (44.79 to 72.91%) towards isolates were shown by penicillin, streptomycin, ampicillin, neomycin, cloxacillin, enrofloxacin, amoxyclav, co-trimaxole, cephalexin. Therefore, for rational use of antibiotics, prior testing of antibiotic sensitivity is suggested.