Induction of TLR-3 expression in IBD virus challenged chickens from genetically different background

The present study was designed to study the role of TLR-3 in relative disease resistance to IBD virus infection in the indigenous Kadaknath and White-leghorn chicken. Macrophages and splenocytes isolated from IBD virus challenged and Poly I:C inoculated birds of both the breeds were analyzed for TLR-3 expression pattern. Macrophages from IBD challenged Kadaknath breed showed maximum of 3.59 fold increase in TLR-3 expression at 24^th hr interval and moderate expression of 2.17 fold at 12^th hr interval as compared to white leghorn. In case of splenocytes, IBD challenged Kadaknath breed showed maximum of 20.11 fold increase in TLR-3 expression at 24^th hr of interval and moderate expression of 5.37 fold at 12^th hr interval. Between the breeds the IBD virus induced TLR-3 expression was more in Kadaknath as compared to White leghorn. In Kadaknath breed the poly I: C could induce only 5 and 2 fold increase of TLR-3 expression in splenocytes at the same intervals as compared to IBD virus, showing natural infection can induce more of TLR-3 expression than synthetic analogs.