1Department of Veterinary Anatomy, Veterinary College and Research Institute, Tirunelveli-627 358, Tamil Nadu, India
2Education Cell, Madras Veterinary College, Chennai-600 007, Tamil Nadu, India
3Department of Veterinary Anatomy, Veterinary College and Research Institute, Udumalpet-642 205, Tamil Nadu, India
4Department of Veterinary Anatomy, Veterinary College and Research Institute, Namakkal-637 002, Tamil Nadu, India
5Department of Veterinary Anatomy, Veterinary College and Research Institute, Orathanadu-614 625, Tamil Nadu, India
6Department of Veterinary Anatomy, Madras Veterinary College, Chennai-600 007, Tamil Nadu, India
*Corresponding Author: S. Rajathi, Department of Veterinary Anatomy, Veterinary College and Research Institute, Tirunelveli-627 358, Tamil Nadu, India, Email: rajathis9936@gmail.com
Online Published on 06 February, 2026.
Pancreatic stellate cell (PSC) is now known to play a critical role in pancreatic fibrosis in chronic pancreatitis and pancreatic cancer. In health, quiescent PSCs maintain normal tissue architecture.
The immunohistochemistry of the pancreas of guinea pig six animals each from 0-2 weeks, 2-8 weeks, 8-16 weeks and 16-32 weeks age groups irrespective of sex were conducted by procuring the animals from the Department of Laboratory Animal Medicine. Tissues were normally processed and embedded in paraffin and paraffin sections of three micron thickness were cut and immunohistochemistry staining protocol was followed with using primary antibody markers namely GFAP, alpha SMA and vimentin.
In guinea pigs of 0-2 weeks of age, quiescent pancreatic stellate cells surrounding the acini showed mild positive vimentin immunoreaction. Mild alpha SMA immunopositive reaction was noticed in the quiescent pancreatic stellate cells of 2-8 weeks, 8-16 weeks and 16-32 weeks of age groups within the pancreatic acini. The star shaped quiescent pancreatic stellate cells in the pancreas were located in the interacinar spaces or periacinar spaces, with elongated cytoplasmic processes. Strong vimentin positive quiescent pancreatic stellate cells were found in the pancreatic acini of 2-8 weeks, 8-16 weeks and 16-32 weeks of age groups.
Guinea pig, Immunohistochemistry, Pancreas, Pancreatic stellate cells
