Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2025
  • Volume: 59
  • Issue: 9

Effect of Different Smoking Methods and Levels of Fat on the Polycyclic Aromatic Hydrocarbon (PAHs) in Buffalo Meat Sausages

  • Author:
  • Anindita Mali1, Saurabh Kumar Laskar1,*, Ankur Das1, Santosh Upadhyay2, Sadhana Choudhury1, Kuleswan Pame3, Protiva Gogoi2
  • Total Page Count: 7
  • Page Number: 1586 to 1592

1Department of Livestock Products Technology, College of Veterinary Science, Assam Agricultural University, Guwahati-781 022, Assam, India

2AICRP on PHET, Department of Livestock Products Technology, College of Veterinary Science, Assam Agricultural University, Guwahati-781 022, Assam, India

3Department of Livestock Products Technology, Lakhimpur College of Veterinary Science, Assam Agricultural University, Guwahati-781 022, Assam, India

*Corresponding Author: Saurabh Kumar Laskar, Department of Livestock Products Technology, College of Veterinary Science, Assam Agricultural University, Guwahati-781 022, Assam, India, Email: saurabhlaskar@gmail.com

Online Published on 06 February, 2026.

Abstract

The study aimed to determine how different smoking methods and levels of fat content affect the generation of polycyclic aromatic hydrocarbons in smoked buffalo meat sausages.

The treatments included conventional smoking at 60°C for 40 minutes (T1A), 3% diluted liquid smoke (T1B) and 7% diluted liquid smoke (T1C). The fat percentage in the sausages was substituted with inulin as a fat replacer in the successive treatments (T2A, T2B, T2C, T3A, T3B, T3C). Nine PAH compounds were analysed: Fluoranthene, chrysene, benzo(a)anthracene, benzo(b) fluoranthene, benzo(k)fluoranthene, indeno(1,2,3-cd)pyrene, benzo(a)pyrene, benzo(ghi)perylene, and dibenzo(a,h)anthracene.

Out of the nine PAHs, fluoranthene and chrysene were identified in the examined treated samples of PAHs. No substantial variance was noted regarding fat levels or smoking methods. The chrysene concentrations varied between 32.27 and 38.37 μg/kg, while fluoranthene levels fluctuated from 15.10 to 53.47 μg/kg. All treated samples were free of benzo(a)pyrene, which is considered the most potent carcinogen.

Keywords

Buffalo meat, Chrysene, Liquid chromatography-mass spectrometry, Polycyclic aromatic hydrocarbons, Sausages, Smoked sausages