Preparation of Porcine Allogenic Acellular Diaphragm Matrix and its Application for Umbilical Hernioplasty in Pig

The surgical reconstruction of large abdominal defects with application of surgical mesh of synthetic origin resulted a number of potential clinical complications; although it is having good tensile strength. Acellular bio-mesh derived from slaughtered animals could be better alternative for surgical management abdominal wall defects. The present study aimed to evaluate bio-detergent based decellularized allogenic porcine acellular diaphragm matrix in umbilical hernioplasty in pig.

For the present study sodium deoxycholate was used as bio-detergent for preparation of porcine allogenic acellular diaphragm matrix (ADM). All total eighteen (18) numbers of piglets aged 2-3 months, body weight 8-14 kg and either sex with umbilical hernia equally divided in to three groups. Group A was subjected to application of ADM; while in Group B injection of methylprednisolone along with ADM application and Autologous Tunica Vaginalis was used for hernioplasty in Group C. Various parameters viz clinical, gross evaluation of surgical wound, haemato-biochemical and indirect ELISA of blood and serum was carried out as per standard methods.

Decellularization protocol carried out with sodium deoxycholate as bio-detergent@ 2% concentration resulted complete decellularization of porcine diaphragm and preserved the distinctive, natural, three-dimensional collagen structure within the prepared matrix. Clinical and haemato-biochemical observations following umbilical hernioplasty in pig with ADM promises its clinical utility. The immune response of host tissue to bio-mesh as revealed by Indirect ELISA was negligible.