1Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India.
*Corresponding Author: J. Jogi, Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Mhow-453 441, Madhya Pradesh, India. Email: drjoycee@gmail.com
Mastitis is mostly caused by mixed infections, of which S. aureus and E. coli are amongst the most important bacteria. Detection of resistant strains of these bacteria is a major public health concern due to the possibility of infection via contaminated milk and milk products intended for human consumption. This study describes simultaneous occurance of methicillin-resistant S. aureus and extended spectrum β-lactamase producing E. coli in mastitic milk samples of buffaloes in Jabalpur, India.
A total of 408 buffaloes milk samples were screened for mastitis by California mastitis test (CMT) test. A total of 102 mastitic milk samples were collected for isolation of Staphylococcus aureus (S. aureus) and Eschirichia coli (E. coli). Polymerase chain reaction was performed for molecular characterization of methicillin resistant S. aureus for mecA gene and ESBL producing E. coli for bla TEM gene, respectively.
On the basis of cultural and biochemical characteristics, 31/102 samples yielded S. aureus and 10/102 samples yielded E. coli. Out of 31 S. aureus isolates, mecA gene was positive in 05 (16.1%) and out of 10 ESBL producing E. coli isolates bla TEM was positive in 02 (20%) isolates. Coexpression of bla TEM and mecA genes was recorded only in 01 (10%) isolate.
Buffaloes, Coexpression, ESBL, Mastitic milk, Polymerase chain reaction
