1Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781 022, Assam, India.
*Corresponding Author: P.M. Barua, Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781 022, Assam, India. Email: prithviraj.barua@gmail.com
Cryopreservation of embryo is a part of most in-vitro fertilization programme. Various cryoprotectants can be used during embryo cryopreservation. As some cryoprotectants are very toxic to embryos therefore exposure of a suitable cryoprotectant at proper concentration and optimum equilibration time during cryopreservation of embryos is of great concern in relation to the survivability of the embryos.
All total 1224 bovine follicular cumulus-oocyte-complexes (COCs) were recovered from 900 abattoir ovaries for the present study. Total 880 numbers of COCs were used to study survivability in Propylene Glycol (PG), Glycerol (GL) and Ethylene Glycol (EG) at 5, 6 and 7 M of concentration and 5, 10 and 15 min of equilibration time.
Out of the three cryoprotectants PG, GL and EG the highest per cent survivability of COCs was recorded in PG at 7 M concentration. In respect of equilibration time the per cent survivability of COCs was found better in 5 min than that of 10 and 15 min.
Bovine, Concentration, Cryoprotectants, Equilibration time, Follicular oocyte
