Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2025
  • Volume: 59
  • Issue: 1

Network pharmacology-based investigation and experimental discovery of Resveratrol's mechanism of liver ischemia reperfusion-induced oxidative stress damage

  • Author:
  • I. Demirhan1,*, E.B. Kurutas2, E. Oner3
  • Total Page Count: 7
  • Page Number: 101 to 107

1Vocational School of Health Services, Harran University, Sanliurfa-63300, Turkiye

2Department of Medical Biochemistry, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras-46050, Turkiye

3Department of Biochemistry, Faculty of Pharmacy, Adiyaman University, Adiyaman-02000, Turkiye

*Corresponding Author: I. Demirhan, Vocational School of Health Services, Harran University, Asst. Prof. Sanliurfa-63300, Turkiye, Email: ilterdemirhan@gmail.com

Online published on 25 June, 2025.

Abstract

This study aimed to investigate the hepatoprotective effect of RSV against oxidative stress damage and we induced an experimental liver Ischemia-reperfusion (I/R) model in rats.

Female albino rats were divided into three groups (n = 8). In contrast to the control group, 1 ml saline (0.09% NaCl) was administered intraperitoneally two days before the operation and after reperfusion in the sham group. 1 ml of 50 mg/kg Resveratrol was administered to the treatment group until two days before the operation. In addition, a single dose of 1 ml of Resveratrol was re-administered after reperfusion. The duration of liver ischemia/reperfusion was determined as 30 minutes in all groups. At the end of this experiment, antioxidant enzyme (CAT, SOD) activities and malondialdehyde (MDA) levels were measured as spectrophotometric in liver tissues homogenates.

Our study observed tissue injuries in the ischemia/reperfusion and Sham groups (p<0.001). This study observed significant decreases in tissue MDA levels in the treatment group compared to the sham and control groups (p<0.001). In addition, antioxidant enzyme levels (SOD. CAT) were significantly increased in the treatment group compared to the control and Sham groups. Liver tissue samples were examined for hepatocyte damage and histopathological grade of the groups were shown. Our study observed tissue injuries in the ischemia/reperfusion and Sham groups (p<0.001). Liver edema, necrosis and PMNL infiltration were found to be decreased in the treatment group compared to the control group. The association of resveratrol with oxidative damage was also determined by network pharmacology analysis.

Keywords

Liver CAT activity, Network analysis, PMNL infiltration, Resveratrol