1The Basic Medical Laboratory of the 920th Hospital of the Joint Logistics Support Force of PLA, Kunming, Yunnan, 650032, China
2The Integrated Engineering Research Center of Cell Biological Medicine of State and Regions, Kunming, Yunnan, 650032, China
3The Transfer Medicine Key Laboratory of Cell Therapy Technology of Yunnan Province, Kunming, Yunnan, 650032, China)
4Department of Medical Information and Data, General Hospital of Southern Theater Command of PLA, Guangzhou, 510010, China
5Queen Mary School, Nanchang University, Nanchang, China
*Corresponding Author: Tao Ye, The Basic Medical Laboratory of the 920th Hospital of the Joint Logistics Support Force of PLA, Kunming, Yunnan, 650032, China, Email: kzkxkyt@126.com
**Xing-Hua Pan, xinghuapan@aliyun.com
Online published on 25 June, 2025.
Natural killer (NK) cells are an important part of the immune system and play a central role in cell-mediated immune responses. Recent studies have shown that NK cells promote organ rejuvenation, thus achieving anti-aging effects from the inside to out.
We amplified and cultured human peripheral blood NK cells and the positive rate reached 43.91%. After purification, the positive rate reached 92.65%. The cultured NK showed a good killing effect on tumor cells and the NK cells reached the standard preparation scale. The NK cells cultured by us were co-cultured with the PBMC of aging macaques and the quantitative PCR and WB results showed that the expression of aging gene and aging protein of the PBMC of aging macaques decreased significantly after co-culture, with statistical significance (P<0.01).
These results indicate that NK cells prepared with standard preparation have anti-aging effect, which will have extensive significance in preclinical research.
Aging gene, Anti-aging, Cell killing experiment, NK cells, Senescence protein, Standardized preparation
