1Department Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia
*Corresponding Author: N. Abutaha, Department Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia, Email: nabutaha@ksu.edu.sa
Online Published on 14 October, 2024.
Breast cancer is a prevalent global health concern. Traditional medicine often incorporates the use of medicinal plants to address various diseases.
The cytotoxicity, oxidative stress and cell migration effects of saponin and phenol extracts were evaluated through MTT assay, ROS analysis and wound-healing assay. Following the identification of the active extract, it underwent GC-MS analysis and in silico investigations.
Our results revealed significant inhibition of cell proliferation in MDA-MB-231 (IC50:407.3 µg/mL) and HUVECs (IC50:500 µg/mL), which was achieved only with the ethyl acetate extract (Fraction 2). Fraction 2 extract induced notable morphological changes and significantly inhibited time-dependent migration in MDA-MB-231 cells. Additionally, it elevated cellular ROS levels compared to the control cells. In molecular docking analysis, out of the 51 chosen secondary metabolites from L. shawii, stigmast-5-en-3-ol, (3α,24S) (-10.0 kcal/mol) and lup-20(29)-ene-3,28-diol (-9.5 kcal/mol) were found to be the best docked to their respective targets-6CHZ and 4MAN, respectively. Therefore, this plant holds promise as a potential therapeutic agent for breast cancer treatment.
6CHZ, Cell migration, Cytotoxicity, MDA-MB-231, Molecular docking
