1ICAR-Directorate of Poultry Research, Hyderabad-500 030, Telangana, India
2ICAR-National Academy of Agricultural Research Management, Hyderabad-500 030, Telangana, India
3School of Agricultural Sciences and Technology, SVKM’s Narsee Monjee Institute of Management Studies (Deemed-to-University), Shirpur-425 405, Maharashtra, India
*Corresponding Author: Jayakumar Sivalingam, ICAR-Directorate of Poultry Research, Hyderabad-500 030, Telangana, India, Email: jeyvet@gmail.com
Online Published on 14 October, 2024.
Microsatellites are short repeat motifs consisting 1-6 base pair (bp) and have a high degree of length polymorphism and are useful to study the genetic diversity. In Chicken, no micro satellite markers were demarcated to different chromosomes, viz., 29, 34, 35, 36, 37, 38 and 39 in the genome. In the present study, we carried out chromosome-wise identification of SSRs that will be of great use in diversity and gene mapping studies in chicken.
The perl script of MISA tool was used to screen the polymorphic microsatellites with different thresholds level.
We identified a total of 397877 SSRs from the latest chicken genome assembly (GRCg7b), of which mononucleotides, dinucleotides, trinucleotides, tetranucleotides, pentanucleotides and hexanucleotides composed of 307453 (77.27%), 40991 (10.30%), 23059 (5.79%), 16723 (4.20%), 8127 (2.04%) and 1524 (0.38%), respectively.
Chicken, Diversity, Genome assembly, Microsatellite markers
