1Department of Veterinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Pakistan
2College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, PR China
3Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, P.O. Box, 2460, Riyadh, 11451, Saudi Arabia
4Institute of Animal and Dairy Sciences, University of Agriculture, Faisalabad, Pakistan
5Department of Education, The University of Lahore, Sargodha Campus, Pakistan
6Center for Advanced Studies and Vaccinology and Biotechnology, University of Balochistan, Quetta, Pakistan
*Corresponding Author: Asghar Ali Kamboh, Department of Veterinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Pakistan, Email: drasgharkamboh@yahoo.com
Online published on 13 August, 2024.
Among life-threatening infections of small ruminants (sheep/goat), enterotoxaemia has vital importance. This disease is caused by certain serotype of Clostridium perfringens and commonly known as pulpy kidney or overeating infection. The current study was carried out to determine the prevalence of C. perfringens in small ruminants in different zones (Kalat, Mangochar, Johan, and Gazg) of district Kalat, Balochistan.
Fecal samples (n=100) were collected aseptically, from rectum of enterotoxaemia suspected animals. The samples were cultured in Reinforced Clostridial Media (RCM). Biochemically identified isolates were further confirmed through polymerase chain reaction (PCR).
Overall prevalence of C. perfringens was noticed 48%, with higher occurrence rate (p < 0.01) in sheep (60%) than goat (36%). Among fifty samples of each specie, 30 and 18 isolates recovered from sheep and goat respectively. The area wise analysis showed that, in goats, the highest prevalence was found in Kalat (50%) followed by the Mangochar (38.46%), Johan (30.77%) and Gazg (25%). The statistical analysis revealed the significant differences (p<0.05; 95% confidence interval (CI) 18.80-68.91) in C. perfringens prevalence in various areas of Kalat district. The results of the gender-wise prevalence of C. perfringens exhibited a numerically higher (p>0.05; 95% CI 9.23-49.02) prevalence in female goats (40%) than male goats (32%). Whereas, the age-wise analysis indicated that, in < 1 year age group (44.44%), the prevalence of C. perfringens was higher (p<0.05; 95% CI 8.00-57.89) than >1 but <2 years (29.41%) and ≥ 2-year (33.33%) age groups. Similarly, in sheep, the highest prevalence (p<0.05) was found in Kalat (76.92%) followed by Mangochar (66.67%), Johan (58.33%) and Gazg (38.46%). The age wise and gender-wise differences (p<0.05) were also observed in various age and gender groups of sheep similar to goat. Among 30 sheep isolates of C. perfringens, a highest number (28 isolates) showed resistance against amikacin, followed by amoxicillin/penicillin (25 isolates) and colistin sulphate (22 isolates); while among 18 isolates of goat 17 were found resistant against colistin sulphate and 16 were resistant against amoxicillin and amikacin. In conclusion enterotoxaemia is prevailing in small ruminants of district Kalat, Balochistan thus mass scale vaccination campaign should be carried out to prevent losses.
Antimicrobial susceptibility, Balochistan, Enterotoxaemia, Goat, Sheep
