1College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781 022, Assam, India
2Department of Zoology, Gauhati University, Guwahati-781 014, Assam, India
*Corresponding Author: S. Basumatary, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781 022, Assam, India, Email: basumatarysuru40@gmail.com
Online published on 17 June, 2025.
The present study compared the quality characteristics of boar semen diluted with three extenders at different hours of preservation.
A total of 24 ejaculates comprising 6 ejaculates from each of four boars of 10-12 months age maintained under uniform management conditions are being selected for the present study. Semen was collected once weekly from four clinically healthy HD-K75 boars by simple fist method. The ejaculated semen was extended in BTS, GEPS and MODENA extender and maintained at 15°C upto 120 hours of preservation. The DNA integrity and the mean fold change in relative expression of HSP70 and Cas3 gene in BTS, GEPS and MODENA extender is also being studied.
The percentage of DNA integrity revealed that no DNA damaged spermatozoa were observed in MODENA extender from 0 to 120 hours of preservation. The relative expression of HSP70 gene elucidated significant difference (P<0.01) at 24 hours in three extenders. The relative expression of Cas3 gene in BTS, GEPS and MODENA extender differed significantly (P<0.01) at 0, 72 and 96 hours of preservation. The overall expression (fold change) of apoptotic gene was found to be higher in BTS extender followed by MODENA and GEPS.
Apoptotic gene, Boar, DNA integrity, Extender, Spermatozoa
