Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2023
  • Volume: 57
  • Issue: 9

Cryoprotective activity of glycerol and ethylene glycol: Dynamics with canine sperm integrity

  • Author:
  • Kaushal Kusum1,*, Ramakrishna Roy2, Vijay B. Sharma3, Raghavendra Prasad Mishra4, Neha Singh5
  • Total Page Count: 6
  • Page Number: 1133 to 1138

1Department of Veterinary Gynaecology and Obstetrics, MJF College of Veterinary and Animal Science, Chomu-303 702, Jaipur, Rajasthan, India

2Dr. Rajendra Prasad Central Agricultural University, Samastipur-848 125, Bihar, India

3Department of Animal Breeding and Genetics, MJF College of Veterinary and Animal Science, Chomu-303 702, Jaipur, Rajasthan, India

4Department of Veterinary Public Health, Apollo College of Veterinary Medicine, Jaipur-302 031, Rajasthan, India

5Department of Veterinary Microbiology, MJF College of Veterinary and Animal Science, Chomu-303 702, Jaipur, Rajasthan, India

*Corresponding Author: Kaushal Kusum, Department of Veterinary Gynaecology and Obstetrics, MJF College of Veterinary and Animal Science, Chomu-303 702, Jaipur, Rajasthan, India, Email: kusumkaushal197@gmail.com

Online published on 30 October, 2023.

Abstract

Different cryoprotectants had been used for safe gaurding the post thaw Canine sperm cell motility. Glycerol and ethylene glycol may be used as cryoprotectants. However, scanty reports are available on use of different concentrations of glycerol, ethylene glycol and the combination of glycerol and ethylene glycol as cryoprotectants for canine spermatatozoa.

In present study, the semen samples were collected from 4 different adult spitz male dogs. The diluted semen was divided into four aliquots, Group I: 8% glycerol (control); Group II: 4% glycerol; Group III: 5% ethylene glycol; Group IV: 4% glycerol + 4% ethylene glycol.

The result showed that post-thaw sperm motility in canine semen with 5% ethylene glycol was recorded as 36.83±1.26%. For Group I (8% glycerol) and Group II (4% glycerol), the same was 34.25±0.95% and 26.50±0.81%, respectively whereas Group IV (4% glycerol + 4% ethylene glycol) it was 29.25%. However, there was no significant correlationship between abnormal sperm count and live spermatozoa; abnormal sperm count and dead spermatozoa; dead spermatozoa and DNA integrity and in between HOST (hypo-osmotic swelling test) and acrosomal integrity of spermatozoa.

Keywords

DNA integrity, Ethylene glycol, HOST, Sperm integrity, Sperm viability