1Department of Veterinary Physiology, West Bengal University of Animal and Fishery Sciences, Kolkata-700 037, West Bengal, India
2Department of Veterinary Anatomy and Histology, West Bengal University of Animal and Fishery Sciences, Kolkata-700 037, West Bengal, India
*Corresponding Author: Dipak Banerjee, Department of Veterinary Physiology, West Bengal University of Animal and Fishery Sciences, Kolkata-700 037, West Bengal, India, Email: dipakndri@gmail.com
Online published on 7 July, 2023.
The present study focused on the localization and comparative expression analysis of RFRP-3 gene in the hypothalamus and testis of adult male rats.
Brain and testis tissue samples were collected from adult Wister rats and used for immunohistochemistry for identification and localization RFRP-3 producing neurons. The expression level of the RFRP-3 gene was assessed by Real-time PCR and compared with muscle tissue.
The results confirmed the presence of RFRP-3 secreting neurons in the hypothalamus and in the testes. Scattered RFRP-3 in cell bodies was identified primarily in the VMH, DMH and surrounding the third ventricle of the brain. In testis, RFRP-3 was detected in Leydig cells, Sertoli cells and germ cells. The expression level of RFRP-3 gene in the hypothalamus was observed to be significantly higher in hypothalamus than in testicular tissue (p<0.01) of adult rats.
Hypothalamus, Immunohistochemistry, Rat, RFRP-3, Testis
