1Veterinary University Training and Research Centre, Tamil Nadu Veterinary and Animal Sciences University, Melmaruvathur-603 319, Tamil Nadu, India
2College of Veterinary Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut-250 110, Uttar Pradesh, India
3ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru- 560 064, Karnataka, India
4Department of Sheep Breeding, Union Trerritory-Ladakh, India
5Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, Uttar Pradesh, India
6Mycobacterium Lab, Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, Uttar Pradesh, India
*Corresponding Author: R. Durairajan, Veterinary University Training and Research Centre, Tamil Nadu Veterinary and Animal Sciences University, Melmaruvathur-603 319, Tamil Nadu, India. Email: duraivet2006@gmail.com
Online published on 17 March, 2023.
Pasteurellosis is an economically important disease of livestock worldwide and vaccination is effective mean to control the disease outbreaks. One of alternative method for vaccine preparation could be the use of lytic phages as bacterial inactivating agents and employing “phage lysate immunogen” as an immunizing agent. Therefore, the study was undertaken to explore the protective immune response of novel phage lysate vaccine against Pasteurella multocida.
A phage lysate vaccine of Pasteurella multocida was prepared using isolated lytic phage and subjected to vaccine response study in rabbits. In this experiment, two mouse fixed-dose of lysate was used and the prophylactic efficacies of lysates were evaluated in rabbits by passive mouse protection test (PMPT), western blotting and direct virulent challenge.
Passive transfer of antibodies to mice using post-immunization sera of rabbits protected the animals against the challenge with A: 1 strain (75%) and B: 2 strain (50%). In western blotting, a total number of 4 bands were observed in the region between 130 kDa to 25 kDa in the protein of the phage lysate. The study suggested the significance increase in humoral immunity by phage lysate in murine Pasteurellosis.
Humoral response, Passive mouse protection assay, Phage lysate, Protective efficacy
