1Uttarakhand Technical University, Sudhowala, Dehradun-248 161, Uttarakhand, India
2Sardar Bhagwan Singh University, Balawala, Dehradun-248 161, Uttarakhand, India
3Vivek College of Technical Education, Bijnor-246 701, Uttar Pradesh, India
*Corresponding Author: Akanksha Awasthi, Vivek College of Technical Education, Bijnor-246 701, Uttar Pradesh, India, Email: akankashaawasthi333@gmail.com
Online Published on 5 January, 2024.
Phytoestrogens have recently become a hot topic among scientists. Phytoestrogens’ estrogen-like properties have led to their widespread use in the reproductive system. The aim of this research was to see whether the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum had any estrogenic activity in female wistar rats.
In female wistar rats, the estrogenic effect was studied using a uterotropic assay, vaginal cytology and vaginal opening. In ovariectomized immature and mature female wistar rats, a 400 mg/kg body weight (b.w.) dose of ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum was given.
When compared to ovariectomized control rats, the uterine wet weight increased significantly. The estrogen-treated rats had only cornified epithelial cells, indicating the existence of oestrogen, as well as 100% vaginal opening. At 400 mg/kg b.w., the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum demonstrated promising estrogenic activity, as evidenced by uterotropic assays, vaginal opening measurements and histopathological changes. As a result of this research, it's possible to infer that the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum play an important role in estrogenic activity in female rats.
Ethanolic extract, Histopathology, Phytoestrogen, Uterotropic assay, Vaginal cytology, Vaginal opening
