1Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar-125 001, Haryana, India
2Department of Animal Production, Faculty of Agriculture, Cairo University, Algammaa Street, 12613, Giza, Egypt
3Cell Biology Department, National Research Center, 12622Dokki, Giza, Egypt
*Corresponding Author: Vinod Chhokar, Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar-125 001, Haryana, India, Email: vinodchhokar@yahoo.com
Online published on 1 December, 2023.
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) is a candidate gene for milk fat in livestock. The present study was carried out to investigate polymorphism in PPARGC1A and study its potential association.
Two hundred forty animals from Murrah and Bhadawari Indian buffalo breeds and Egyptian buffaloes were selected. The statistical analysis used was Tukey's honest significance test to investigate the association of different genotypes. The PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) assays and sequencing technologies were used to confirm the SNPs.
Three Novel single nucleotide polymorphisms (SNP1- g.993A>T, SNP2- g.1237T>A and SNP3-g.1238G>C) and one insertion (g.1240≥G bp) were identified in this gene. Due to nucleotide mutations, four non-synonymous mutations were observed in amino acid sequences of buffalo breeds. Significant differences in protein and SNF output were discovered in Egyptian buffalo breeds. In both Murrah and Bhadawari buffaloes, fat, protein and SNF yield were substantially correlated with detected genotypes while there was non-significant association of protein and SNF per cent in buffalo breeds. The information provided will be valuable in studying the involvement of PPARGC1A in regulating fat synthesis and enhancing milk quality in buffaloes.
Bubalus bubalis, PCR-SSCP, PPARGC1A, SNPs
