1College of Animal Science and Technology, Henan Key Laboratory of Unconventional Feed Resources Innovative Utilization, Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China
2College of Veterinary Medicine, Henan Key Laboratory of Unconventional Feed Resources Innovative Utilization, Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China
3School of Animal Science, Yangtze University, Jingzhou, 434020, China
*Corresponding Author: D.F. Jiang, College of Animal Science and Technology, Henan Key Laboratory of Unconventional Feed Resources Innovative Utilization, Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China. Email: 120232625@qq.com
Online published on 30 September, 2022.
Intestinal health of livestock and poultry is the basis of their body health. Different expression of growth factors is important cause of intestinal dysfunction, which further induced the illness of livestock and poultry. S-Methylmethionine Sulfonium Chloride (SMMSC) is also the main metabolite of methionine in animals. However, there are few studies exploring the relationship between SMMSC and intestinal epithelial growth factors.
Piglet jejunal epithelial cells IPEC-J2 were treated with different concentrations of SMMSC at 0.1, 0.5 and 1 mM for 24 h respectively. Then real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting (WB) were employed to detect the expression of Mucin-2 (MUC2), epidermal growth factor (EGF), transforming growth factor beta (TGF-p,), glucagon-like peptide-2 (GLP-2) and insulin-like growth factor-1 (IGF-1).
The results showed that 0.5 and 1 mM of SMMSC could stimulate the expression of MUC2, EGF, GLP-2 and IGF-1 while inhibit the expression of TGF-β1 in both mRNA and protein level (p<0.05). It is of great significance to repair intestinal mucosa injury, maintain intestinal mucosa barrier and promote intestinal development.
Grow factors, Intestinal mucosal epithelium, IPEC-J2, MUC2, SMMSC
