1Department of Veterinary Gynaecology and Obstetrics, West Bengal University of Animal and Fishery Sciences, Kolkata-700 037, West Bengal, India.
2Department of Microbiology, Mrinalini Datta Mahavidyapith, Birati, Kolkata-700 051, West Bengal, India.
3Stem Cell Laboratory, Department of Veterinary Clinical Complex, West Bengal University of Animal and Fishery Sciences, Kolkata-700 037, West Bengal, India
*Corresponding Authors: Kalyani Ray, Department of Veterinary Gynaecology and Obstetrics, West Bengal University of Animal and Fishery Sciences, Kolkata-700 037, West Bengal, India, Email: kalyaniray2@gmail.com
**Shyamal Kanti Guha, Stem Cell Laboratory, Department of Veterinary Clinical Complex, West Bengal University of Animal and Fishery Sciences, Kolkata-700 037, West Bengal, India, Email: drskguha2004@yahoo.co.in
Online Published on 09 May, 2022.
Few studies have confirmed the presence of ovarian tissue stem cells indicating the capacity for differentiation. Based on this fact, it was hypothesized that mesenchymal stem cells (MSC) were found in ovarian surface epithelium (OSE) of canines that could easily be isolated.
Both left and right ovaries were minced and digested using collagenase to obtain a stromal vascular fraction (SVF). MSCs were characterized using RT-PCR. To ascertain the trilineage differentiation potential, MSCs were stained with respective stain for osteocytes, chondrocytes and adipocytes.
We observed elongated, spindle-shaped and fibroblast like appearance of cells after 72 h of initial culture. Expression of MSC specific surface markers were observed through RT-PCR. Using Stem Pro® differentiation medium, OSE were differentiated into osteogenic, chondrogenic and adipogenic lineages and were found to be potential source for isolation, characterization and differentiation of MSCs. Canine (OSE) is easily accessible, multipotent and has high plasticity, holding promise for applications in regenerative medicine.
Canine ovarian surface epithelium, Mesenchymal stem cells, Ovariectomy, Stromal vascular fraction, Trilineage differentiation
