Clinico-pathological and hemato-biochemical assessment of field originated chicken anemia virus in experimentally challenged broiler chicken

One hundred broiler chicks were divided into two groups A and B. The chicks in group A were control group and group B was challenged with field originated chicken infectious anemia virus (CIAV) at 7^th day of age. The samples were collected on 7^th, 14^th, 21^st, 28^th and 35^th day of post infection for hematological, biochemical, immunohistochemical and histopathological examination. The hematological analysis showed significantly (P<0.05) low level of hemoglobin, decreased packed cell volume, leukocytopenia and thrombocytopenia in infected group as compared to control group. In immunohistochemical assay, CIAV was detected in portal zone of liver, epicardium and myocardium, lymphoid follicles of bursa of Fabricius, thymic lobules and mainly white pulp of spleen. Histopathological changes were observed in both lymphoid and non-lymphoid organs. Concerning the serum biochemical profile, hypoproteinemia, hypoalbuminemia, increased concentration of liver enzymes, creatinine and urea were observed in infected birds. Our results showed that field originated stra in is pathogenic for broilers which induce significant changes in hematology, serum biochemical profile and histopathology of lymphoid and non-lymphoid organs.