1Division of Animal Health, ICAR Research Complex for NEH Region, Umiam-793 103, Meghalaya, India
2College of Veterinary and Animal Science, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut-250 110, Uttar Pradesh, India
*Corresponding Author: Samir Das, Division of Animal Health, ICAR Research Complex for NEH Region, Umiam-793 103, Meghalaya, India, Email: drsamirvph@yahoo.com
Online published on 22 July, 2021.
Mycobacteroides chelonae M77 isolated in a targeted Mycobacterial isolation study from bovine raw milk was whole genome sequenced (CP041150.1), followed by comparative genomics study for better understanding of the organism with special focus on its virulence and antibiotic resistance pattern. This is probably the first whole genome sequence (WGS) of M.cheloane submitted in NCBI database from India.
The WGS has been carried out in Illumina Next Seq 500 platform and then assembled resulting in a genome of 5.12 MB. The assembled genome was annotated in RASTtk and viewed by SEED for its detailed analysis. Pathogen Finder 1.1 and Res Finder 3.1 soft wares were applied for knowing the isolate pathogenecity and antibiotic profiling respectively. Mauve analysis was carried out to know the genomic re-arrangements.
Pathogen Finder 1.1 showed that M77 was non-pathogenic and Res Finder 3.1 server reveals it is susceptible to most of the antibiotics. Mauve analysis for alignment of isolate revealed no significant re-arrangement of genomic segment. The whole analysis showed that the genome of M77 is tight genome with not much variation in relation to previous complete genomes reported and is non-pathogenic and sensitive to most of the antibiotics.
Mycobacteroides chelonae, Nontuberculous mycobacteria, Tuberculosis, Whole genome sequencing
