1Shandong Collaborative Innovation Center for Development of Veterinary Pharmaceuticals, College of Veterinary Medicine, Qingdao Agricultural University, Qingdao-266 109, China
2Shandong Lvdu Biotechnology Co. Ltd.Binzhou-256 600, China
Ministry of Agriculture Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Diseases, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot-010 018, China
*Corresponding Author: Yongjun Wen, Hu Shan; Ministry of Agriculture Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Diseases, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot-010 018, China
**Shandong Collaborative Innovation Center for Development of Veterinary Pharmaceuticals, College of Veterinary Medicine, Qingdao Agricultural University, Qingdao-266 109, China, Email: yongjunwen@126.com; Email: shanhu67@163.com
Online published on 22 July, 2021.
Canine distemper virus (CDV) can infect a variety of predators, such as Canidae, Mustelidae and Procyonidae family and cause high morbidity and mortality. Genetic/antigenic heterogeneity has been observed among various CDV strains, notably in the haemagglutinin (H) gene. Based on sequence analysis of the H gene, wild-type CDV strains can be divided into distinct genotypes.
We used Vero/SLAM cells to isolate CDV strains from clinically naturally infected dog tissues. Here, the H gene sequence of the isolated strain was analyzed for the restriction enzyme polymorphism with RT-PCR-RFLP method and the method could be used to distinguish CDV vaccine strains from wild-type strains. Furthermore, the genetic and antigenic variation of the H gene was also analyzed.
Our results indicated that Vero cells expressing canine SLAM allow the efficient isolation of CDV wild-type strains. The isolated CDV was named Shandong strain. Phylogenetic analysis identified that Shandong strain was CDV wild-type strain, the Asian genotype I. Analysis of the potential glycosylation site of the H gene and determination of the neutralization titer PD50 showed that there were immunogenic differences between the Shandong strain and the vaccine strains.
Canine distemper virus, Haemagglutinin gene, Isolation, Phylogenetic analysis
