1College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, P.R.China
2Heilongjiang Key Laboratory of Efficient Utilization of Feed Resources and Nutrition Manipulation in Cold Region, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, P.R.China
3Jingchun Li and Yanbing Li contributed equally to this paper
*Corresponding Author: Guosheng Wei and Jingchun Li, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, P.R.China, Email: elj863@163.com
Online published on 22 July, 2021.
The present study was aimed to investigate the effects of negative pressure applied before storage on the gene expression of motility related proteins in boar spermatozoa.
Boar semen samples were collected and pooled and diluted with Modena solution containing 0.4% (w/v) of bovine serum albumin. Negative pressure was applied for 2–5 min using a vacuum pump with a barometer. The pressure applied was 0 (Control), -0.02 MPa (P2), -0.04 MPa (P4) and -0.08 MPa (P8). The expression of AQN-1, AQN-3, AWN, PSP-I, PSP-II gene in boar spermatozoa was evaluated.
Application of -0.04 MPa improved the sperm motility compared with the other groups. In conclusion, our results confirmed thatnegative pressure preservation at 170C had an effect on the expression of boar sperm adhesion protein gene. The relative expression of AWN0PSP-I and PSP-II genes in boar sperm were lower under -0.04 MPa and -0.08 MPa pressure, which was beneficial to protect spermatozoa motility.
Boar semen, Gene expression, Motility related proteins, Negative pressure
