1College of Animal Science, Jilin University, Changchun, Jilin, 130 062, China
2Five-Star Animal Health Pharmaceutical Factory of Jilin Province, Changchun, Jilin, 130 062, China
3School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200 240, China
4College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, 510 642, China
*Corresponding Author: Linlin Hao, Ying Zhang, College of Animal Science, Jilin University, Changchun, Jilin, 130 062, China, Email: haolinlin@jlu.edu.cn
Online published on 15 May, 2021.
The transcript variants of Insulin-like growth factor 1 receptor (IGF-1R) and their expression profiles had never been illuminated in pigs until now. Herein, we identified IGF-1R AS02 as a novel splice variant of IGF-1R gene by RT-PCR and analyzed its mRNA expression level by qRT-PCR in liver, cartilage and muscle tissues, while also detecting the single-nucleotide polymorphism (SNP) site near the splice site of the IGF-1R gene (in intron 19) of BaMa and Landrace pigs. Results demonstrated that the IGF-1R AS02 variant showed a significantly (P<0.05) higher expression level in cartilage than in muscle and liver across two pig breeds respectively. The expression level of the normal transcript (IGF-1R ISO01) of IGF-1R in cartilage was markedly lower than that in the other two tissues (P<0.05). In cartilage, IGF-1R ISO01 expression was higher in BaMa than in Landrace (P<0.05), while the expression level of IGF-1R AS02 was lower in BaMa than in Landrace (P<0.05). The SNP was detected in intron 19 of the IGF-1R gene of BaMa and Landrace pigs. These results contributed to facilitating a better understanding of IGF-1R gene in pigs.
Alternative splicing, IGF-1R gene, Pig, Tissue expression
