Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 004, Haryana, India
*Corresponding Author: Y. Singh, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 004, Haryana, India, Email: y.singhjadon@yahoo.com
Online published on 15 May, 2021.
Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the two most pathogenic avian mycoplasmas. In the present study, examination of 92 pooled tissue samples from broiler chicken of 92 different poultry flocks of Haryana (India) exhibiting respiratory infections resulted in isolation of 13 (14%) Mollicutes. Based on biochemical reactions, growth inhibition test, PCR and/or sequencing, 8 (8.6%) isolates could be characterized as MG, 1 (1.08%) as MS, 3 (3.24%) as M. gallinarum and 1 (1.08%) as Acholeplasma laidlawii. The phylogenetic analysis using Intergenic spacer region (IGSR) of these MG isolates revealed that they clustered with USA strain whereas the vaccine strains were in different clade. Single locus sequence typing (SLST) revealed considerable nucleotides variation between 8 MG isolates and vaccine strains. Conclusively, Sequencing of IGSR region of MG can be used as a valuable epidemiological investigation tool for the differentiation of wild-type MG strains from vaccine strains.
Avian mycoplasmosis, Isolation, Mycoplasma gallisepticum, Mycoplasma synoviae, Nucleotide sequencing
