1Department of Veterinary Microbiology, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India
ESBL Department of Veterinary Pharmacology and Toxicology, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India
*Corresponding Author: Arpita Shrivastav, Department of Veterinary Pharmacology and Toxicology, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India, Email: arpitavet@gmail.com
Online published on 15 May, 2021.
Bacterial resistance to beta-lactam antibiotics has risen dramatically in Escherichia coli from poultry and other food animals due to the production of extended-spectrum beta-lactamase enzyme (ESBLs) which degrades third generation cephalosporins.Herbs could be a better alternative for such cases.
Present study was undertaken on 400 caecal samples of healthy broilers, collected from various poultry sale outlets of Jabalpur (M.P.). Samples were screened phenotypically and genotypically for the presence of extended spectrum beta lactamase producing E. coli. Inhibitory effect of fruit peel juice of Punica granatum and Syzigium aromaticum oil observed on the Beta lactamase enzyme obtained from these positive samples by colorimetric method using CENTA and NITROCEFIN as chromogenic substrate.
Phenotypically 135 samples/isolates were ESBL producing E. coli. On multiplex PCR assay 76 positive samples with blaTEM, blaCTX and blaSHV, gene was obtained. Oil of Syzygium aromaticum showed maximium per cent inhibition, Punica granatum depicted lower per cent inhibition with CENTA and NITROCEFIN respectively. Combination of both herbs showed increased inhibition. Tazobactum, (100μM) taken as the standard control exhibited 99.88 and 98 per cent inhibition of ESBL enzyme.
Antimicrobial resistance, Beta lactamase, BlaCTX, BlaSHV, BlaTEM, Escherichia coli, Extended spectrum, Poultry
