1College of Animal Science and Technology, Henan University of Science and Technology, Luoyang-471 003, China
2Mutton Sheep Breeding Engineering Technology Research Center of Henan Province, Luoyang-471 003, China
3Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing-100 193, China
*Corresponding Author: Yuqin Wang, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang-471 003, China, Email: wangyq6836@163.com
Online published on 20 July, 2020.
To further to understand the structure and function of the TAC1 gene, we cloned the full-length cDNAs of the TAC1 genes from goat by rapid amplification of cDNA ends-PCR and the qRT-PCR was used to analyze the TAC1 mRNA expression patterns of goat various tissues. The full-length cDNA of goat TAC1 was 1176 bp, with a 339 bp open reading frame encoding 112 amino acids. The amino acid sequence analysis revealed that goat TAC1 gene encoded a water-drain protein and its relative molecular weight and isoelectric point was 13,012.86 Da and 6.29 respectively. Alignment and phylogenetic analyses revealed that their amino acid sequences were highly similar to those of other vertebrates. TAC1 expression of the goat of the brain, cerebellum, medulla oblongata, heart, liver, spleen, lung, kidney, uterus, ovaries. These results serve as a foundation for further study on the Capra hircus TAC1 gene.
Clone and sequence analysis, Expression, Goat, Tachykinin
