Investigating immunomodulating activities of recombinant horse IL-2, IL-18 and IFN-γ in peripheral blood mononuclear cells (PBMCs)

Recombinant horse cytokines may be used as biological adjuvant for enhancing vaccine efficacy and therapeutic agent. This study describes low cost production of recombinant horse IL-2, IL-18 and IFN-γ in prokaryotic system. Immunomodulatory activities of recombinant horse IL-2, IL-18 and IFN-γ have been demonstrated in horse peripheral blood mononuclear cells (PBMCs). Recombinant horse IL-2 and IL-18 enhanced cellular proliferation and significantly up regulated transcription and secretion of IFN-γ, TNF-α, IL-4, IL- 10 and IL-12p35. In contrast, recombinant horse IFN-γ was comparatively less active and did not show significant immune-stimulatory effect.