Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2019
  • Volume: 53
  • Issue: 4

Sequencing and Polymorphism detection in growth hormone gene in Najdi Sheep and their association with milk production

  • Author:
  • Mohamed Afifi1,3,6, Ali Alkaladi1, Naif M. Kadasa1,2, Salina Saddick2, Mohammed Baeshen1, Ammar Al-Farga4,, Faisal M. Aqlan5, Yaaser Q. Almulaiky4, Mohammed Alkhaled1, Aisha A.M. Alayafi1, Haytham A. Ali3,4, Mohiuddin Khan Warsi4, Ayed S. Allogmani7, Aaser Abdelazim3,8
  • Total Page Count: 6
  • Page Number: 423 to 428

1Department of Biological Sciences, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia

2Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia

3Department of Biochemistry, Faculty of Veterinary Science, Zagazig University, Egypt

4Department of Biochemistry, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia

5Department of Chemistry, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia

6University of Jeddah Center for Scientefic and Medical research, Jeddah, Saudi Arabia

7Department of Biological Sciences, Faculty of Science, University of Jeddah, khulais branch, Jeddah, Saudi Arabia

8Department of Basic Medical Sciences, College of Applied Medical Sciences, University of Bisha, Bisha, Saudi Arabia

Department of Biochemistry, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia

*Corresponding author's e-mail: abdalwhap.a737@gmail.com

Abstract

This study was aimed to evaluate the relationship between growth hormone (GH) gene polymorphism and milk production in Najdi sheep. Two hundred Najdi ewes were used to determine the daily milk yield (DMY). The complete CDS of the Najdi sheep GH gene is1950 in length, GenBank acc. no. KU310891.1. Najdi sheep GH protein consists of 217 amino acids GenBank ALT22407.1 with conversion amino acid arginine number 121 toaminoacid lysine (R121K). A novel five SNPs were detected in Najdi sheep GH gene (G871A, A1259C, G1383A, A1486G and A1509G). A significant association between the detected SNPsgeno types and daily milk yield was found in this study. The regression analysis indicated thepositive effect of genotype and SNPs on DMY. Individuals carrying homozygote mutant alleles had the highest DMY. Consequently, these SNP may be useful indicators in the selection of ewes for milk production.

Keywords

Growth hormone gene, Milk production, Najdi sheep, SNPs